疾患モデル動物解析学分野｜東京医科歯科大学

2025年

• Identification of Gallbladder-Specific Distal Regulatory Sequence of Murine Sox17. Shihan Zeng, Ayaka Yanagida, Noriaki Ota, Mami Uemura, Yoshikazu Hirate, Ryuji Hiramatsu, Naoaki Mizuno, Yoshiakira Kanai , Masami Kanai-Azuma. Genes Cells. 2025 Jan;30(1):e13186. doi:10.1111/gtc.13186.

• Versatile Application of Fast Green FCF as a Visible Cholangiogram in Adult Mice to Medium-Sized Mammals. Tomoyuki Niimi, Nanae Miyazaki, Hironobu Oiki, Mami Uemura, Shihan Zeng, Watcharapon Promsut, Noriaki Ota, Hajime Takei, Hiroshi Nittono, Seiko Narushima, Ayaka Yanagida, Ryuji Hiramatsu, Masami Kanai-Azuma, Shohei Takami, Jun Fujishiro, Yoshiakira Kanai. Scientific Reports

2024年

• Impact of Gallbladder Hypoplasia on Hilar Hepatic Ducts in Biliary Atresia. Nanae Miyazaki, Shohei Takami, Mami Uemura, Hironobu Oiki, Masataka Takahashi, Hiroshi Kawashima, Yutaka Kanamori, Takako Yoshioka, Mureo Kasahara, Atsuko Nakazawa, Mayumi Higashi, Ayaka Yanagida, Ryuji Hiramatsu,  Masami Kanai-Azuma, Jun Fujishiro & Yoshiakira Kanai.  Communications Medicine 4 Article number:111, June 2024. https://doi.org/10.1038/s43856-024-00544-5

• Skin graft with dermis and appendages generated in vivo by cell competition. Nagano, H., Mizuno, N., et al. Nature Communications volume 15, Article number: 3366, 2024. https://doi.org/10.1038/s41467-024-47527-7

• Multiple ageing effects on testicular/epididymal germ cells lead to decreased male fertility in mice. Tsutomu Endo, Kiyonori Kobayashi, Takafumi Matsumura, Chihiro Emori, Manabu Ozawa, Shimpei Kawamoto, Daisuke Okuzaki, Keisuke Shimada, Haruhiko Miyata, Kentaro Shimada, Mayo Kodani, Yu Ishikawa-Yamauchi, Daisuke Motooka, Eiji Hara, Masahito Ikawa. Communications Biology 2024 Jan; 7, 16

2023年

• Age-related decline in spermatogenic activity accompanied with endothelial cell senescence in male mice. Manabu Ozawa, Hideto Mori, Tsutomu Endo, Yu Ishikawa-Yamauchi, Daisuke Motooka, Chihiro Emori, Masahiro Ikawa. iScience 2023 Dec; 26(12) 108456-108456

• Chronic non-discriminatory social defeat stress during the perinatal period induces depressive-like outcomes in female mice. Masumi Ito, Hikaru Ito, Kaori Miyoshi, Masami Kanai-Azuma. Brain Res Jan 2024  1825:148734. doi: 10.1016/j.brainres.2023.148734.
• Accumulation of senescent cells in the stroma of aged mouse ovary. Natsumi Maruyama, Isuzu Fukunaga, Tomoaki Kogo, Tsutomu Endo, Wataru Fujii, Masami Kanai-Azuma, Kunihiko Naito, Koji Sugiura. Journal of Reproduction and Development Oct 6 2023 69(6)
• A Sox17 downstream gene Rasip1 is involved in the hematopoietic activity of intra-aortic hematopoietic clusters in the midgestation mouse embryo. Gerel Melig,Ikuo Nobuhisa,Kiyoka Saito,Ryota Tsukahara,Ayumi Itabashi,Yoshiakira Kanai,Masami Kanai-Azuma,Mitsujiro Osawa,Motohiko Oshima,Atsushi Iwama,Tetsuya Taga. Inflammation and Regeneration  Aug 8 2023 43:41,doi:org/10.1186/s41232-023-00292-4
• A small secreted protein NICOL  regulates lumicrine-mediated sperm maturation and male fertility. Daiji Kiyozumi, Kentaro Shimada, Michael Chalick, Chihiro Emori, Mayo Kodani, Seiya Oura, Taichi Noda, Tsutomu Endo, Martin M Matzuk, Daniel H Wreschner, Masahito Ikawa. Nature Communications 2023 Apr 24, 14, 2354. doi: 10.1038/s41467-023-37984-x

• ※ A possible function of Nik-related kinase in labyrinth layer of mouse placentas of delayed delivery. Hiroshi Yomogita, Hikaru Ito, Kento Hashimoto, Akihiko Kudo, Toshiaki Fukushima, Tsutomu Endo, Yoshikazu Hirate, Yoshihiro Akimoto, Masayuki Komada, Yoshiakira Kanai, Naoyuki Miyasaka, Masami Kanai-Azuma. Journal of Reproduction and Development Feb 2023 69(1)
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2022年

• SOX17-positive rete testis epithelium is required for Sertoli valve formation and normal spermiogenesis in the male mouse. Aya Uchida, Kenya Imaimatsu, Honoka Suzuki, Xiao Han, Hiroki Ushioda, Mami Uemura1, Kasane Imura-Kishi, Ryuji Hiramatsu, Hinako, M. Takase, Yoshikazu Hirate, Atsuo Ogura, Masami Kanai-Azuma, Akihiko Kudo, Yoshiakira Kanai. Nature Communications(online:2022 Des 22.)doi:10.1038/s41467-022-35465-1
• PDCL2 is essential for sperm acrosome formation and male fertility in mice. Yoshitaka Fujihara, Kiyonori Kobayashi, Ferheen Abbasi,Tsutomu Endo, Zhifeng Yu, Masahiro Ikawa, Martin M Matzuk. Andrology 2022 Oct 24. doi:10.1111/andr.13329. Online ahead of print.

• A Review of Delayed Delivery Models and the Analysis Method in Mice. Hiroshi Yomogita, Naoyuki Miyasaka, Masami Kanai-Azuma. J Dev Biol 2022 May 20;10(2):20. doi: 10.3390/jdb10020020.

2021年

• MAB21L1 modulates gene expression and DNA metabolic processes in the lens placode. Ryuichi Yamada, Akira Oguri, Katsunori Fujiki, Katsuhiko Shirahige, Yoshikazu Hirate, Masami Kanai-Azuma, Hirotaka Takezoe, Yoshihiro Akimoto, Naoki Takahashi, Yoshiakira Kanai. Disease Models & Mechanisms 2021 Dec 1;14(12):dmm049251. doi: 10.1242/dmm.049251. Epub 2021 Dec 23.

• WNT signaling in pre-granulosa cells is required for ovarian folliculogenesis and female fertility. Okiko Habara, Catriona Y Logan, Masami Kanai-Azuma, Roeland Nusse, and Hinako M Takase. Development 2021 May 1 ;148(9):dev198846. doi: 10.1242/dev.198846.

• Early crypt formation defects in the uterine epithelia of Sox17-heterozygous mice. Hirate Y,Hayakawa K,Nakano Y,Kumazawa S,Miura K,Kanai Y,Kanai-Azuma M. Sexual Dev 2021 Mar 9;1-11. doi: 10.1159/000513386
• Low Retinoic Acid Levels Mediate Regionalization of The Sertoli Valve in The Terminal Segment of Mouse Seminiferous Tubules. Imura-kishi K,Uchida A,Tsunekawa N,Suzuki H,M.Takase H,Hirate Y,Kanai-Azuma M,Hiramatsu R,kurohmaru M&Kanai Y. Sci Rep 2021 Jan 13;11(1):1110. doi: 10.1038/s41598-020-79987-4
  
• MKK7-deficiency in mature neurons impairs parental behavior in mice. Shin T,Hiraoka Y, Yamasaki T, Marth JD, Penninger JF, Kanai-Azuma M, Tanaka K, Kofuji S, Nishina H. Genes to Cells 2021 Jan;26(1):5-17. doi: 10.1111/gtc.12816. Epub 2020 Nov 18.

2020年

• Activation of proprotein convertase in the mouse habenula causes depressiove-like behaviors through remodeling of extracellular matrix. Ito H, Nozaki K, Sakimura K, Abe M, Yamawaki S, Aizawa H.　Neuropsychopharmacology
• Dopaminergic signaling in the nucleus accumbens modulates stress-coping strategies during inescapable stress. Cui W, Aida T, Ito H, Kobayashi K, Wada Y, Kato S, Nakano T, Zhu M, Isa K, Kobayashi K, Isa T, Tanaka K, Aizawa H.　Journal of Neuroscience
• Antidepressant effect of the translocator protein antagonist ONO-2952 on mouse behaviors under chronic social defeat stress. Nozaki K, Ito H, Ohgidani M, Yamawaki Y, Sahin EH, Kitajima T, Katsumata S, Yamawaki S, Kato TA, Aizawa H.　Neuropharmacology, 162:107835. 2020.
• Anatomical and histological characteristics of the hepatobiliary system in adult Sox17 heterozygote mice. Pattarapanawan M, Uemura M, Miyazaki N, Takami S, Tomiyasu H, Tsunekawa N, Hirate Y, Fujishiro J, Kurohmaru M, Kanai-Azuma M, Higashiyama H, Kanai Y.　　Anat Rec (Hoboken)　2020 Dec;303(12):3096-3107. doi: 10.1002/ar.24466. Epub 2020 Jul 2.
• Gallbladder wall abnormality in biliary atresia of mouse Sox17^+/− neonates and human infants. Uemura M, Higashi M, Pattarapanawan M, Takami S, Ichikawa N, Higashiyama H, Furukawa T, Fujishiro J, Fukumura Y, Yao T, Tajiri T, Kanai-Azuma M, Kanai Y.　　Disease Models & Mechanisms 2020 : dmm.042119 doi: 10.1242/dmm.042119 Published 29 January 2020
• Heterozygous mutation of the splicing factor Sf3b4 affects development of the axial skeleton and forebrain in mouse. Yamada T, Takechi M, Yokoyama N, Hiraoka Y, Ishikubo H, Usami T, Furutera T, Taga Y, Hirate Y, Kanai-Azuma M, Yoda T, Ogawa-Goto K, Iseki S.　　Dev Dyn. 2020 Jan 3. doi: 10.1002/dvdy.148.

2019年

• Phospholipase C-related catalytically inactive protein regulates lipopolysaccharide-induced hypothalamic inflammation-mediated anorexia in mice. Yamawaki Y, Shirawachi S, Mizokami A, Nozaki K, Ito H, Asano S, Oue K, Aizawa H, Yamawaki S, Hirata M, Kanematsu T.　Neurochemistry International, 131:104563. 2019.
• Endocardium differentiation through sox17 expression in endocardium　precursor cell regulates heart development in mice.Saba R,Kitajima K,Rainbow L,Engert S, Uemura M, Ishida H,Kokkinpoulos L,Shintani Y, Miyagawa S, Kanai-Azuma M, Koopman P, Meno C, Kenny J, Lickert H, Kanai Y, Suzuki K, Saga Y, Yashiro K　　　　Sci Rep. 6;9(1):11953. doi: 10.1038/s41598-019-48321-y. (IF 4.0)
• Nr5a1 suppression during the fetal period optimizes ovarian development by fine-tuning of Notch signaling.Nomura R, Kashimada K, Suzuki H, Zhao L, Hosokawa-Tsuji A, Yagita H, Takagi M, Kanai Y, Bowles J, Koopman P, Kanai-Azuma M, and Morio T. 　　 J Cell Sci   Apr 15;132(8) 2019

• Molecular and genetic characterization of partial masculinization in embryonic ovaries grafted into male nude mice. Miura K, Harikae　K, Nakaguch M, Imaimatsu K, Hiramatsu R, Tomita A, Hirate Y, Kanai-Azuma M, Kurohmaru M, Ogura A, Kanai Y.　　　PLOS ONE　　14(3):e0212367　　2019

2018年

• Sox17 is Essential for Proper Formation of the Marginal Zone of Extraembryonic Endoderm Adjacent to a Developing Mouse Placental Disk. Igarashi H, Uemura M, Hiramatsu R, Hiramatsu R, Segami S, Pattarapanawan M, Hirate Y, Yoshimura Y, Hashimoto H, Higashiyama H, Sumitomo H, Kurohmaru M, Saijoh Y, Suemizu H, Kanai-Azuma M, Kanai Y. 　Biol Reprod. 　99(3):578-589  2018
• Maintenance of hematopoietic stem and progenitor cells in fetal intra-aortic hematopoietic clusters by the Sox17-Notch1-Hes1 axis. Saito K, Nobuhisa I, Harada K, Takahashi S, Anani M, Lickert H, Kanai-Azuma M, Kanai Y, Taga T.  Exp Cell Res.  365(1):145-155  2018
• Mouse GTSF1 is an essential factor for secondary piRNA biogenesis. Yoshimura T, Watanabe T, Kuramochi-Miyagawa S, Takemoto N, Shiromoto Y, Kudo A, Kanai-Azuma M, Tashiro F, Miyazaki S, Katanaya A, Chuma S, Miyazaki J.  EMBO Reports  e42054  2018
• Differentiation of ovaries. Takase MH, Kanai-Azuma M, Kanai Y.  Reference Module in Biomedical Sciences. Elsevier.  in press  2018
• Anatomy and development of the extrahepatic biliary system in mouse and rat: a perspective on the evolutionary loss of the gallbladder. Higashiyama H, Uemura M, Igarashi H, Kurohmaru M, Kanai-Azuma M and Kanai Y.  J Anat. 　232(1):134-145.  2018

2017年

• Spermatogonial deubiquitinase USP9X is essential for proper spermatogenesis in mice. Kishi K, Uchida A, Takase HM, Suzuki H, Kurohmaru M, Tsunekawa N, Kanai-Azuma M, Stephen A. Wood and Kanai Y.  Reproduction.  154(2):135-143.  2017
• Embryonic Cholecystitis and Defective Gallbladder Contraction in the Sox17-haploinsufficient Model of Biliary Atresia.
  Higashiyama H, Ozawa A, Sumitomo H,  Uemura M, Fujino K, Igarashi H, Imaimatsu K, Tsunekawa N, Hirate Y, Kurohmaru M, Saijoh Y, Kanai-Azuma M and Kanai Y.  Development  144(10):1906-1917.  2017
• Defects in the first wave of folliculogenesis in mouse XO ovaries.
  Miura K, Murata C, Harikae K, Suzuki H, Kanai-Azuma M, Kurohmaru M, Tsunekawa N, Kanai Y.　 J Reprod Dev. 63(3):333-338.  2017
• Essential roles of L-type amino acid transporter 1 in syncytiotrophoblast development by presenting fusogenic 4F2hc.
  Ohgaki R, Ohmori T, Hara S,Nakagomi S, Kanai-Azuma M, Kaneda-Nakashima K, Okuda S, Nagamori S, Kanai Y.  Mol Cell Biol.  e00427-16.  2017

2016年

• In vivo dynamics of GFRα1-positive spermatogonia stimulated by GDNF signals using a bead transplantation assay.
  Uchida A, Kishi K, Aiyama Y, Miura K, Takase HM, Suzuki H, Kanai-Azuma M, Iwamori T, Kurohmaru M, Tsunekawa N, Kanai Y.  Biochemical and Biophysical Research Communications.  476(4):546-52.  2016
• Mouse Sox17 haploinsufficiency leads to female subfertility due to impaired implantation.
  HirateY, Suzuki H, Kawasumi M, Takase HM, Igarashi H, Naquet P, Kanai Y, Kanai-Azuma M. Scientific Reports. 　6:24171 　2016
• Paracrine Wnt/β-catenin signaling mediates proliferation of undifferentiated spermatogonia in the adult mouse testis.
  Takase HM, Nusse R. 　PNAS. 　113(11):E1489-97. 　2016

2015年

• From Sex Determination to Initial Folliculogenesis in Mammalian Ovaries: Morphogenetic Waves along the Anteroposterior and Dorsoventral Axes.
  Suzuki H, Kanai-Azuma M, Kanai Y. 　Sex Dev. 　9(4):190-204. 　2015
• Lhx8 regulates primordial follicle activation and postnatal folliculogenesis.
  Ren Y, Suzuki H, Jagarlamudi K, Golnoski K, McGuire M, Lopes R, Pachnis V, Rajkovic A.  BMC Biol.  13:39. doi: 10.1186/s12915-015-0151-3.  2015
• A Niche for GFRα1-positive spermatogonia in the Terminal Segments of the Seminiferous Tubules in Hamster Testes.
  Aiyama Y, Tsunekawa N, Kishi K, Kawasumi M, Suzuki H, Kanai-Azuma M, Kurohmaru M, Kanai Y.  Stem Cells.  33(9):2811-2824.  2015
• Fate Mapping of Gallbladder Progenitors  in Posteroventral Foregut Endoderm of Mouse Early Somite-stage Embryos.
  Uemura M, Igarashi H, Ozawa A, Tsunekawa N, Kurohmaru M, Kanai-Azuma M, Kanai Y.  J Vet Med Sci.  77(5):587-591.  2015
• Par-aPKC-dependent and -independent mechanisms cooperatively control cell polarity, Hippo signaling, and cell positioning in 16-cell stage mouse embryos.
  Hirate Y, Hirahara S, Inoue K, Kiyonari H, Niwa H, Sasaki H. 　Dev Growth Differ. 　57(8):544-56. 　2015

2014年

• Induction of the G2/M transition stabilizes haploid embryonic stem cells.
  Takahashi S, Lee J, Kohda T, Matsuzawa A, Kawasumi M, Kanai-Azuma M, Kaneko-Ishino T and Ishino F.  Development.  141(20):3842-3847.  2014
• A novel AMH-Treck transgenic mouse line allows toxin-dependent loss of supporting cells in gonads.
  Shinomura M, Kishi K, Tomita A, Kawasumi M, Kanezashi H, Kuroda Y, Tsunekawa N, Ozawa A, Aiyama Y, Yoneda A, Suzuki H, Saito M, Picard JY, Kohno K, Kurohmaru M, Kanai-Azuma M, Kanai Y.  Reproduction.  148(6):H1-9.  2014
• Spermatogonial SOHLH1 nucleocytoplasmic shuttling associates with initiation of spermatogenesis in the rhesus monkey (Macaca mulatta).
  Ramaswamy S, Razack BS, Roslund RM, Suzuki H, Marshall GR, Rajkovic A, Plant TM.  Mol Hum Reprod.  20(4):350-357.  2014
• Sox17-mediated maintenance of fetal intra-aortic hematopoietic cell clusters.
  Nobuhisa I, Osawa M, Uemura M, Kishikawa Y, Anani M, Harada K, Takagi H, Saito K, Kanai-Azuma M, Kanai Y, Iwama A, Taga T.  Mol Cell Biol.  34(11):1976-1990.  2014
• The transition from stem cell to progenitor spermatogonia and male fertility requires the SHP2 protein tyrosine phosphatase.
  Puri P, Phillips BT, Suzuki H, Orwig KE, Rajkovic A, Lapinski PE, King PD, Feng GS, Walker WH.  Stem Cells.  32(3):741-753.  2014
• Syntaxin 1B, but not syntaxin 1A, is necessary for the regulation of synaptic vesicle exocytosis and of the readily releasable pool at central synapses.
  Mishima T, Fujiwara T, Sanada M, Kofuji T, Kanai-Azuma M, Akagawa K.  PLoS One.  9(2):e90004.  2014
• HIPPO pathway members restrict SOX2 to the inner cell mass where it promotes ICM fates in the mouse blastocyst.
  Wicklow E, Blij S, Frum T, Hirate Y, Lang RA, Sasaki H, Ralston A. PLoS Genet. 10(10):e1004618. 2014

2013年

• Targeted Gene Deletion of miRNAs in Mice by TALEN System.
  Takada S, Sato T, Ito Y, Yamashita S, Kato T, Kawasumi M, Kanai-Azuma M, Igarashi A, Kato T, Tamano M, Asahara H.  PLoS One.  8(10):e76004.  2013
• Heterogeneity in sexual bipotentiality and plasticity of granulosa cells in developing mouse ovaries.
  Harikae K, Miura K, Shinomura M, Matoba S, Hiramatsu R, Tsunekawa N, Kanai-Azuma M, Kurohmaru M, Morohashi K, Kanai Y.  J Cell Sci.  126(Pt13):2834-2844.  2013
• Targeted gene silencing in mouse germ cells by insertion of a homologous DNA into a piRNA generating locus.
  Yamamoto Y, Watanabe T, Hoki Y, Shirane K, Li Y, Ichiiyanagi K, Kuramochi-Miyagawa S, Toyoda A, Fujiyama A, Oginuma M, Suzuki H, Sado T, Nakano T, Sasaki H.  Genome Res.  23(2):292-299.  2013
• Sox17 haploinsufficiency results in perinatal biliary atresia and hepatitis in C57BL/6 background mice.
  Uemura M, Ozawa A, Nagata T, Kurasawa K, Tsunekawa N, Nobuhisa I, Taga T, Hara K, Kudo A, Kawakami H, Saijoh Y, Kurohmaru M, Kanai-Azuma M, Kanai Y.  Development.  140(3):639-648.  2013
• Generation of a germ cell-specific mouse transgenic CHERRY reporter, Sohlh1-mCherryFlag.
  Suzuki H, Dann CT, Rajkovic A.  Genesis.  51(1):50-58.  2013

2012年

• Dynamics of GFRα1-positive spermatogonia at the early stages of colonization in the recipient testes of W/W(vv) male mice.
  Nagai R, Shinomura M, Kishi K, Aiyama Y, Harikae K, Sato T, Kanai-Azuma M, Kurohmaru M, Tsunekawa N, Kanai Y.  Developmental Dynamics.  241(8):1374-1384.  2012
• Gut endoderm is involved in transfer of left right asymmetry from the node to the lateral plate mesoderm in the mouse embryo.
  Saund RS, Kanai-Azuma M, Kanai Y, Kim I, Lucero MT, Saijoh Y.  Development.  139(13):2426-2435.  2012
• Expression of matrix metalloproteinases 2 and 9 and tissue inhibitors of matrix metalloproteinases 2 and 1 in the glomeruli of human glomerular diseases: the results of studies using immunofluorescence, in situ hybridization, and immunoelectron microscopy.
  Sekiuchi M, Kudo A, Nakabayashi K, Kanai-Azuma M, Akimoto Y, Kawakami H, Yamada A.  Clin Exp Nephrol.  16(6):863-874.  2012
• SOHLH1 and SOHLH2 coordinate spermatogonial differentiation.
  Suzuki H, Ahn HW, Chu T, Bowden W, Gassei K, Orwig K, Rajkovic A.  Dev Biol.  15;361(2):301-312.  2012

2011年

• Cyclical and patch-like GDNF distribution along the basal surface of Sertoli cells in mouse and hamster testes.
  Sato T, Aiyama Y, Ishii-Inagaki M, Hara K, Tsunekawa N, Harikae K, Uemura-Kamata M, Shinomura M, Zhu XB, Maeda S, Kuwahara-Otani S, Kudo A, Kawakami H, Kanai-Azuma M, Fujiwara M, Miyamae Y, Yoshida S, Seki M, Kurohmaru M, Kanai Y.  PLoS One.  6(12):e28367.  2011
• Sox17-dependent gene expression and early heart and gut development in Sox17-deficient mouse embryos.
  Pfister S, Jones VJ, Power M, Truisi GL, Khoo PL, Steiner KA, Kanai-Azuma M, Kanai Y, Tam PP, Loebel DA.  Int J Dev Biol.  55(1):45-58.  2011
• Proteomic analysis of two types of exosomes in human whole saliva.
  Ogawa Y, Miura Y, Harazono A, Kanai-Azuma M, Akimoto Y, Kawakami H, Yamaguchi T, Toda T, Endo T, Tsubuki M, Yanoshita R.  Biol Pharm Bull.  34(1):13-23.  2011